FP6 priority
1.1.3   Nanotechnologies and Nanosciences, Knowledge-based Multifunctional Materials and New Production Processes and Devices
Title of the proposal

Development of the biosensors on the solid support.

Slovak Academy of Sciences, Institute of Animal Biochemistry and Genetics
Moyzesova 61, 900 24 Ivanka pri Dunaji, Slovak Republic
Maja SNEJDARKOVA, Ing. Csc.,
+421 2 45943151

Research subject for a potential FP6 project

Monitoring the environment for the presence of compounds which may adversely affect human health and local ecosystem is a fundamental part of a regulation to maintain a habitable environment. The adoption of new materials and new principles of transduction techniques play an important role in the process of improving the characteristic of existing instruments and in the development of new measurement systems. One possible way could be the use of biosensors. The analytical application of biologically important enzymes offers broad possibilities for the developing of new techniques of analytical monitoring.
In our laboratory we have constructed the biosensor based on the biotinylated phospholipid and streptavidin – modified glucose oxidase for the estimation of glucose. The direct amperometric immunosensor for the detection of antigen – antibody interaction on the gold support modified by γ-chain specific anti human IgG was checked. Immobilization of avidin–modified acetylcholin-esterase and choline oxidase on a metal-supported biotinylated membrane allows to develop a minisensor for detection of environmental pollutants. The peculiarities of glucose biosensors based on different generation of supramolecular structure such as dendrimers have been studied by amperometry and QCM techniques. This study shown that stable glucose biosensor can be obtained with low generation of dendrimers.

Recent international cooperation of the research team

New Mexico State University, Las Cruces, USA; University of Athens, Greece; State University Kazan, Russia; State University, Budapest, Hungary; Polish Academy of Sciences, Poland; State University, Cambridge, England;

Proposer´s relevant publications related to the research subject

Snejdarkova M., Rehak M., Otto M.: Design of glucose minisensor based on streptavidin – glucose oxidase complex coupling with self – assembled biotinylated phospholipid membrane on solid support. Analytical Chemistry 1993, 65, 665 / 668.
Rehak M., Snejdarkova M., Hianik T.: Acetylcholine minisensor based on metal-supported lipid bilayers for determination of environmental pollutants. Electroanalysis 1997, 9, 1072 –1077.
Hianik T.,Snejdarkova M.,Sokolikova L., Meszar E., Krivanek R.,Tvarozek V.,Novotny I.,Wang J.: Immunosensor based on supported lipid membranes, protein films and liposomes modified by antibodies. Sensor and Actuators 1999, 57, 201 –212.
Snejdarkova M., Svobodova L.,Gajdos V.,Hianik T.: Glucose biosensor based on dendrimer monolayers. J. Mater. Science 2001, 12, 1079 – 1082.